Process of producing a remedy for tuberculosis.



vextracted.

WILHELM RUPPEL, OF Ht jCHST-ONTHE-M AI-N.

GERMANY, ASSIGNOR TC FARBWERKE VORM. MEISTER-LUCIUS 8c BBUNING. 0F HGCHST-ON-THEMAIN, GERMANY; A Q011- PORATION OF (SiEIiJlIIANY. I

PROCESS OF 1.?RODUCING A REMEDY FOR TUBERCULOSIS.

Specification of Letters Patent.

Patented Jan. 14,1913.

No Drawing. Original application filed July 18, 1910, Serial No. 572,638. Divided and this application tiled September 26, 1911. Serial No 651.401.

'1}; all IF/IO/it it may conccrn:

Be it known that I, 'lVl'L-i-mrn R'UPPBL, Ph. D. and professor of medicine a citizen of the Empire of Germany, residing at Hiichst-on-tbe-l[aim Prussia,of the Empire of Germany, have invented certain new and useful Improvements in Processes of Pro-a dncing a Remedy for Tubercplosis, of which the following is a specification.

Specific all lltltbQl'cLllOSlS-SEIUIU. such as for instance that. obtained according to my U. S. patent application Serv No. 572,638, tiled July 18, l9 it), contains nnmune' bodies which act on tnberclobacilli and other preparations derived therefrom. As the treatment of tuberculosis in the St rum-tl'ierapcutical, way always oti'ers 'ditlitulties by complications arising in the incorporation of albumins. it. seemed advisable toisolate the immune bodies from the serum. In this respect tubercle-bacilli are loaded With the serum, whereby combination of the immune bodies -t v'tnh tnberclwliacillitakes place 'loetnat etlect. it was first ascertaine'cFby the usual methids what amount of the various specific immune bodies were contained in theantitubereulosis-sernm tobe pose it is sullicient to determine as exactly as possible the agglutinatingpower of the serum and to find by calculation the quanlily of serum lVlllt'll is able to act on a (ten taiu amonut of tubercle-lau-illi. Thus. for instance, we proceed as follows: 1 gr. of

completiagglutination of the said quantity" of lullel't'ltklltltilllh, if, for instance, 0.003

gr. of this tnlmrclmbacilli are completely agglutinated by 0.0035 c. c. of serum, then corresponding quantities of tubercle-bacilli and antituberculosis'serum, preferably an excess of the latter, are used. for the preparation of the remedy. In a similar manner the amount of complementfixing sub it was found that for this pur stance, ops-onins or bacteriotropins contained lit the tlntlttibtIC1ll0SlS-SltlIll ascertained. Also by this preliminary test' the best combination of antitubereulo-' sis-serum and tuberclobacilli can be determined. The mixture of immune antitubercle-serum and tubercle-bacilli combined according to the foregoing preliminary. test are allowed to stand in an incubator at; 3725? C. for A to *8 hours. The bacilli are thereby loaded with immune bodies and rendered non-toxic. Then they are shaken together with glass beads in a' shaking apparatus urlzl no intact tubercledricilli can be microscopically detected. \Vhcn the llllbCl'ClC-btla Cllll are so far altered, they are separated from the superfluous serum by ccntrifugating. To thesetnberclobacilli physiological salt solution and glycerin. are added in the proportion of for instance, 121200, to form a fine emulsion. 'liuw emulsions of sensitized tubercle-bacilii are used as a. remedy for tuberculosis. It hae been proved that tuberculous patients stand injections thereof without any febrile reaction and obtain permanent improvemem'.

This application is a division of my specified prior one, Ser. No. 572,638.

Having thus described the nature of my invention what I claim is: V

The process of producing emulsions of sensitized tubercle-bacilli as a remedy for. tuberculosis, consisting in causing a specific antitnberculosisserum to act upon tubercle bacilli, so that the tubt t'tltdJtlCllll are ren" dered nontoxic and loaded with speciticin nuine bodies, shaking the mixture of sensitizcd tubercle-bacilli and serum for a long time with insoluble inert hard material in a shaking apparatus until the bacilli are pe fectly triturated, separating serum and bacilli by centrifugating. and mixing the isolated bacilli with suitable quantities of salt solution and glycerin, a as to form an opalescent milky liquid.

in testimony whereof, I altix my signature. in presence of two witnesses.

JEAN Grimm), CARL GRUND. 

